Subject: Re: CCL:K+ channel

From: Eberhard von Kitzing <[email-masked]>
Date: Wed, 12 Jan 2000 08:44:24 +0100

Dear Daniele Spera,

>is anyone aware of existing PDB (or other format) file about the =
>morphological structure of membrane-cell calcium-controlled potassium =
>channel with low conductance? Can anyone point me to some references?

At the moment I know only about a single structure of a
potassium channel. However, it is only a part of the channel.
The part responsible for gating is missing:

HEADER    POTASSIUM CHANNEL                       23-JUL-98   1BL8

TITLE     POTASSIUM CHANNEL (KCSA) FROM STREPTOMYCES LIVIDANS

COMPND    MOL_ID: 1;

COMPND   2 MOLECULE: POTASSIUM CHANNEL PROTEIN;

COMPND   3 CHAIN: A, B, C, D;

COMPND   4 FRAGMENT: RESIDUES 1 - 125;

COMPND   5 ENGINEERED: YES;

COMPND   6 MUTATION: L90C;

COMPND   7 BIOLOGICAL_UNIT: HOMOTETRAMER

SOURCE    MOL_ID: 1;

SOURCE   2 ORGANISM_SCIENTIFIC: STREPTOMYCES LIVIDANS;

SOURCE   3 EXPRESSION_SYSTEM: ESCHERICHIA COLI;

SOURCE   4 EXPRESSION_SYSTEM_STRAIN: XL-1 BLUE;

SOURCE   5 EXPRESSION_SYSTEM_PLASMID: PQE60;

SOURCE   6 EXPRESSION_SYSTEM_GENE: KCSA

KEYWDS    POTASSIUM CHANNEL, INTEGRAL MEMBRANE PROTEIN

EXPDTA    X-RAY DIFFRACTION

AUTHOR    D.A.DOYLE,J.M.CABRAL,R.A.PFUETZNER,A.KUO,J.M.GULBIS,

AUTHOR   2 S.L.COHEN,B.T.CHAIT,R.MACKINNON

REVDAT   1   29-JUL-98  1BL8   0

JRNL        AUTH   D.A.DOYLE,J.M.CABRAL,R.A.PFUETZNER,A.KUO,

JRNL        AUTH 2 J.M.GULBIS,S.L.COHEN,B.T.CHAIT,R.MACKINNON

JRNL        TITL   THE STRUCTURE OF THE POTASSIUM CHANNEL: MOLECULAR

JRNL        TITL 2 BASIS OF K+ CONDUCTION AND SELECTIVITY

JRNL        REF    SCIENCE                       V. 280    69 1998

JRNL        REFN   ASTM SCIEAS  US ISSN 0036-8075

==========================================================

Eberhard von Kitzing

Abteilung Zellphysiologie
Max-Planck-Institut fuer Medizinische Forschung
Jahnstr. 29
D 69120 Heidelberg        Tel: +49 6221 486 467
Germany                   FAX: +49 6221 486 459

email: [email-masked]
WWW: http://sunny.mpimf-heidelberg.mpg.de/people/vkitzing/