From uucp@scnit.saratov.su Wed Feb 21 05:48:32 1996 Received: from sequent.kiae.su for uucp@scnit.saratov.su by www.ccl.net (8.7.1/950822.1) id EAA07249; Wed, 21 Feb 1996 04:50:10 -0500 (EST) Received: by sequent.kiae.su id AA15521 (5.65.kiae-2 for CHEMISTRY@www.ccl.net); Wed, 21 Feb 1996 12:37:34 +0300 Received: by sequent.KIAE.su (UUMAIL/2.0); Wed, 21 Feb 96 12:37:33 +0300 Received: by scnit.saratov.su (UUPC/@ v6.14d, 30Mar95); id AA30254 Tue, 20 Feb 1996 12:58:28 +0300 To: CHEMISTRY@www.ccl.net Message-Id: Organization: Saratov State University From: "polyakov" Date: Tue, 20 Feb 96 12:58:27 +0300 X-Mailer: BML [MS/DOS Beauty Mail v1.36h] Lines: 3 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Please subscribe me on CCL Vadim I.Polyakov e-mail:polyakov@scnit.saratov.su From MAILER-DAEMON@www.ccl.net Wed Feb 21 08:48:40 1996 Received: from gate.texaco.com for MAILER-DAEMON@www.ccl.net by www.ccl.net (8.7.1/950822.1) id IAA08538; Wed, 21 Feb 1996 08:46:26 -0500 (EST) Received: by gate.texaco.com id AA00592 (Mail SMTP Gateway 3.0 for chemistry@www.ccl.net); Wed, 21 Feb 1996 07:46:26 -0600 Message-Id: Date: 21 Feb 1996 07:18:07 GMT From: "MSBEA01.SUBRAPS" Subject: Dipole-Dipole Interaction in Dynamics To: chemistry@www.ccl.net Comment: MEMO 1996/02/21 07:25 ---------------------------------------------------------------------- THIS MAIL MESSAGE IS FROM THE INTERNET AND MAY HAVE BEEN READ, COPIED, OR MODIFIED BY USERS OTHER THAN INTENDED RECIPIENTS. ---------------------------------------------------------------------- Dear List Members, Is there a Molecular Dynamics Package which incorporates dipole-dipole interaction in the electrostatic computation?. I would appreciate any input in this regard. Thanks --Mani-- Dr. P. S. Subramanian Fuels and Lubrication Division Texaco Inc. P. O. Box 509 Beacon, NY 12508 email: SUBRAPS@texaco.com vmail: 914-838-7561 From owner-chemistry@ccl.net Wed Feb 21 11:14:23 1996 Received: from bedrock.ccl.net for owner-chemistry@ccl.net by www.ccl.net (8.7.1/950822.1) id KAA09749; Wed, 21 Feb 1996 10:18:47 -0500 (EST) Received: from ipsun.ras.ru for alne@ibch.siobc.ras.ru by bedrock.ccl.net (8.7.1/950822.1) id KAA29353; Wed, 21 Feb 1996 10:18:27 -0500 (EST) Received: from siobc.siobc.ras.ru by ipsun.ras.ru with SMTP id AA11863 (5.67a/IDA-1.4.4 for ); Wed, 21 Feb 1996 18:18:46 +0300 Received: from ibch.siobc.ras.ru (ibch [194.85.9.2]) by siobc.siobc.ras.ru (8.6.12/8.6.12) with ESMTP id SAA19536 for ; Wed, 21 Feb 1996 18:19:16 +0300 Received: from localhost by ibch.siobc.ras.ru (8.6.5/) id RAA02481; Wed, 21 Feb 1996 17:30:49 +0300 Message-Id: <199602211430.RAA02481@ibch.siobc.ras.ru> Date: Wed, 21 Feb 1996 18:11:25 From: "Nekrasov Alex" Organization: ibch To: chemistry@ccl.net X-Mailer: MAIL2 ( Ver. 2.03.1B from TECHNO ) X-Protocol: POP3 Subject: research project Dear Collegues: We would like to collaborate with you and submit a joint research proposal to U.S. Civilian Research and Development Foundation (WWW: http://www.internext.com/crdf). We look forward to beginning the dialogue and then to expanding our research and business relations. If you are interested in cooperation in these mutual investigation we will greatly appreciate you kindly reply by FAX or E-mail. Please do not reply to the mailing list because we are not subscribed. Dr. A. Wulfson, Ph.D. Head of the Recombinant Proteins Group FAX: +7 (095) 330-7274 E-mail: wan@ibch.siobc.ras.ru _____________________________________________________________________________ General: our research group of biotechnology of recombinant proteins deals with isolation, purification and production of biologically active human recombinant proteins such as cytokines and insulin. Recently we carried out the development of a technological scheme for the production of human TNF-alpha (wild-type as well as mutants), Il-3 and IFN-gamma. Based upon strains designed at our Institute, we are developing at present the technological operation for production of insulin. Results of our investigation of TNF-alpha will be published in Russian Journal of Bioorganic Chemistry N3, 1996 (English edition is available) "An Effective Method of Purification For Human Recombinant Tumor Necrosis Factor Alpha". R.V. Tikhonov, S.A. Yakimov, V.G. Korobko, A.N. Wulfson Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871, Miklukho-Maklaya St. 16/10, Moscow, Russia. During the progress of our research work we have faced some problems connected with preservation of biological activity of rhIFN-gamma. That's only one reason why we have no opportunity to start the clinical trials of our product and make promotion of the medicines based on rhIFN-gamma for clinical applications. To solve this problem we are going to make experimental work to design more stable variants of rhIFN-gamma in the following lines of investigation: 1. COMPUTER DESIGN OF PROTEINS The computer modeling by the molecular dynamics method of protein spatial structure to determine potential mutation regions which allow formation of rhIFN-gamma dimer binding by S-S bonds. 2. GENETIC ENGINEERING a) Preparation of N-terminal deletion mutants lacking 7-10 residues. We suppose that such shortening will increase the stability of protein and facilitate its renaturation without lost of protein activity b). Preparation of mutant analogs of rhIFN-gamma in which the spatial structure would be stabilized by intermolecular S-S bonds. 3. STRUCTURAL ANALYSIS Analysis of the protein dimer spatial structure by X-ray analysis or NMR-spectroscopy. 4. BIOTECHNOLOGY Isolation and purification of the recombinant protein, its renaturation and determination of reagents and buffer solutions in which the protein remains stable during long time periods and can be lyophilized with minimal lost of activity. We hope that a combination of these approaches would give us the possibility to design stable substance of protein rhIFN-gamma for our further work. From polowin@hyper.hyper.com Wed Feb 21 13:48:37 1996 Received: from www.hyper.com for polowin@hyper.hyper.com by www.ccl.net (8.7.1/950822.1) id NAA10927; Wed, 21 Feb 1996 13:11:55 -0500 (EST) Received: from hyper.hyper.com (hyper.hyper.com [204.50.97.9]) by www.hyper.com (8.6.12/8.6.12) with SMTP id NAA06809; Wed, 21 Feb 1996 13:23:45 -0500 Received: by hyper.hyper.com (920330.SGI/920502.SGI) for @www.hyper.com:CHEMISTRY@www.ccl.net id AA18627; Wed, 21 Feb 96 13:22:27 -0500 Date: Wed, 21 Feb 96 13:22:27 -0500 From: polowin@hyper.hyper.com (Joel Polowin) Message-Id: <9602211822.AA18627@hyper.hyper.com> To: dsmith@debye.dasgroup.com (Doug Smith), CHEMISTRY@www.ccl.net Subject: Re: CCL:problem running Hyperchem 4.5 > From: dsmith@debye.dasgroup.com (Doug Smith) > Date: Tue, 20 Feb 1996 23:10:07 +0000 > > I have version 4.5 running on my Pentium, along with ChemPlus 1.5. I have > just changed from Windows 3.1 to Windows NT Workstation 3.51. Now Hyperchem > does not find the hardware lock, and looks on the network for a network > license (which doesn't exist). How can I quickly fix this problem? For HyperChem to communicate with the hardware lock under Windows NT, it is necessary to install an NT port driver. That driver is available via our WWW page (http://www.hyper.com ) under Support / FAQs, or from our ftp site (ftp.hyper.com) in the support directory. The file is rainport.exe, and it is a self-extracting archive file that should be executed with the '-d' option to restore the directory structure of the archive: rainport -d It contains README files that describe the installation procedure. The driver is, of course, also available by writing to support@hyper.com . Joel ------------ Joel Polowin, Ph.D. Manager, Scientific Support Email to: polowin@hyper.com Hypercube Inc, 419 Phillip St, Waterloo, Ont, Canada N2L 3X2 (519)725-4040 Info requests to: info@hyper.com Support questions to: support@hyper.com Email group: Send "subscribe hyperchem" to hyperchem-request@hyper.com WWW: http://www.hyper.com/