AMP and GMP concentrations: summary

From: Irilenia Nobeli <nobeli %-% at %-% biochemistry.ucl.ac.uk>
Subject: AMP and GMP concentrations: summary
Date: Thu, 8 Feb 2001 16:49:12 +0000 ("GMT)

 Many thanks to James Smith and Doug Markham for their answers to my
 question regarding relative concentrations of AMP and GMP in a cell.
 I include the original answers, in case anyone is interested.
 Irilenia
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 > From James.Smith %-% at %-% ddg.phar.cam.ac.uk  Fri Feb  2 09:53:36 2001
 Purine nucleotides vary in their concentration, due to the localised
 release and  turnover. Local high concentrations may also depend on local
 divalent cation concentrations (that chelate phosphate groups).
 Remember eurkaryotic cells are compartmentalised and their organisation is
 dependent on their specialisation but even oocytes (often used as
 expression systems) don't have  homogenous cytoplasms.
 If you receive concentration values for intracellular/extracellular purine
 monophosphate, purine cyclic-monophosphate, purine di and tri phosphate
 purines be very cautious of the cell type, the method and conditions used.
 If you have values from different sources in different cell types, the
 relative values may be more important than the absolute values.
 Yours sincerely
 James Smith
 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
 > From gd_markham %-% at %-% fccc.edu  Thu Feb  1 21:07:57 2001
   There is a long list of nucleotide concentrations in
 Salmonella in :
 B.R. Bochner and B.N. Ames, J. Biol. Chem. Vol. 257, p. 9759-9769 (1982)
 Hope this helps,
 Doug Markham