temperature for MD of a protein



 Hi,
 thanks for all the replies to my previous question. I resolved the issue
 with abrormal bond energies.
 I have a question regarding the temperature at which MD needs to run for a
 protein. I am new to PDB files, so this might be a trivial question. The
 starting coordinates to simulate a protein are taken from the PDB.
 1.How does one decide at what temperature the MD run needs to be done?
 2.In other words, experimentally, when a PDB structure is determined, is
 temperature a relevant factor?
 3. would the structure be different if done at a different temperature?
 4. is the temperature information encoded in the PDB file?
 Thanks
 vani