CCL: IC50 deltaG

From: "Elaine Meng" <meng],[cgl.ucsf.edu>
Subject: CCL: IC50 deltaG
Date: Mon, 2 Jun 2008 12:16:27 -0400

 Sent to CCL by: "Elaine  Meng" [meng+*+cgl.ucsf.edu]
 Hello,
 The Cheng-Prusoff equation is used to calculate K(i) from IC50, when IC50 is
 from a competition assay.  It factors in the concentration and affinity of the
 competing ligand.
 See for example:
 http://www.unmc.edu/Pharmacology/receptortutorial/competition/analysis_sample4.htm
 http://www.tocris.com/PharmGlossUS.php
 Elaine
 -----
 Elaine C. Meng, Ph.D.                          meng%x%cgl.ucsf.edu
 UCSF Computer Graphics Lab and Babbitt Lab
 Department of Pharmaceutical Chemistry
 University of California, San Francisco
                      http://www.cgl.ucsf.edu/home/meng/index.html