CCL: Protein structures



Hi John, 

Paper by Kuzmanic et al also may be of interest for you:

"X-ray refinement significantly underestimates the level of microscopic heterogeneity in biomolecular crystals" Nature Communications 5, Article number: 3220 (2014)
http://www.nature.com/articles/ncomms4220

Regards,
Arthur 

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чт, 5 янв. 2017 г. в 8:47, Lars Goerigk lars.goerigk#unimelb.edu.au <owner-chemistry==�.net>:
Hi John,

not sure if this is helpful as a starting point, but an analysis of common methods for geometry optimisations in periodic boundary conditions, incl. a comparison with a crystal structure and free R-factors, has been published in

J. Phys Chem B 2014, 118, 14612-14626. Therein you can also find a literature review and a summary of the work by Ryde and Thiel in the area of quantum refinement of  protein X-ray structures with QM/MM.

Best wishes,

Lars


Dr Lars Goerigk | Lecturer & ARC DECRA Fellow
School of Chemistry | The University of Melbourne
Victoria 3010 | Australia
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On 5 Jan 2017, at 8:42 am, Jim Kress jimkress35.-$-.gmail.com <owner-chemistry-$-ccl.net> wrote:

> Ignoring hardware and software demands for the moment is it preferable/practical/wise to optimize such a structure?
 
It depends on whether it is intrinsically disordered or not.  It also depends on whether you want an in vivo representation or if you think the in-vacuo representation is representative of the actual structure in vivo.
 
Jim
 
From: owner-chemistry+jimkress35==gmail.com]![ccl.net [mailto:owner-chemistry+jimkress35==gmail.com]![ccl.net] On Behalf Of John McKelvey jmmckel*gmail.com
Sent: Wednesday, January 04, 2017 12:17 PM

To: Kress, Jim <jimkress35]![gmail.com>
Subject: CCL: Protein structures
 

Folks,

I realize that over time development of technology may have an impact on reliability or accuracy of protein XRAY structures.  How does one determine accuracy or reliability of such structures?  Ignoring hardware and software demands for the moment is it preferable/practical/wise to optimize such a structure?

Many thanks!

John

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